Frequency of the HLA-DRB1 and HLA-DRQ1 alleles in Mexican women with oncogenic HPV infection

Authors

  • Díaz-Chiguer DL Indianilla Specialty Clinic, Institute of Security and Social Services for State Workers, City México, Mexico.
  • Andrade-Almaraz V Regional Hospital “Centenario de la Revolución Mexicana”, Institute of Security and Social Services for State Workers, Morelos, México.
  • Domínguez-Velazco H Regional Hospital “Centenario de la Revolución Mexicana”, Institute of Security and Social Services for State Workers, Morelos, México.
  • Orihuela- Orihuela J Regional Hospital “Centenario de la Revolución Mexicana”, Institute of Security and Social Services for State Workers, Morelos, México.
  • Torres- Mejía IA Regional Hospital “Centenario de la Revolución Mexicana”, Institute of Security and Social Services for State Workers, Morelos, México.
  • Loera-Piedra AA Indianilla Specialty Clinic, Institute of Security and Social Services for State Workers, City México, Mexico.
  • Sanchez-Aleman M Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.
  • Chavez- Cardenas M Consejo Nacional de Ciencia y Tecnología (CONACyT)-Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.
  • Mondragon-Teran P Centro Médico Nacional 20 de Noviembre, Institute of Security and Social Services for State Workers, Mexico City, Mexico.

Abstract

Objective: The purpose of this study was to determine the expression of E6 / E7 mRNA of HPV-16 and HPV-18, along with the presence of HLA II-DRB1 and DQB1 variants in a Mexican population.

Methods: The women who attended to the primary services in Morelos, Mexico, during 2019 were invited to participate in the conventional cytology and biomolecular testing for the detection of HPV. The mRNA assay method for detection of mRNA from 14 different HR-HPV using target capture of E6 and E7. Low-resolution PCR-sequence-specific priming (SSP) was used for amplification of HLA DR-DQ alleles in both cases.

Results: A total forty-eight women studies were included. The prevalence of HPV E6/E7 mRNA was 50%, and distributed as follows: 20% patients with the presence of E6/E7 mRNA from HPV-16, 24% patients with the presence of E6/E7 mRNA from HPV-18/45 genotype and 6% patients with the presence of E6/E7 mRNA from other HR-HPV. The most frequent allele in HPV-positive cases (32%;8/25) was DRB1*01 for the HLA DRB1 locus, followed by the HLA DRB1*04 allele (26%;13/48). It should be noted that for the group of HPV positive patients, DRB1*015 was detected, but not in the group of patients without HPV. Discussion: In this study, alleles HLA-DRB1*15 and HLA-DRB1*04 were identified in women infected by oncogenic HPV, but were not detected in absence of HPV infection. These findings are consistent with reports for populations of Asian, Caucasic, and Latin-American women, which suggests that this allele is a prognostic marker for CC development.

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Published

2023-04-17

How to Cite

DL, D.-C., V, A.-A., H, D.-V., J, O.-. O., IA, T.-. M., AA, L.-P., M, S.-A., M, C.-. C., & P, M.-T. (2023). Frequency of the HLA-DRB1 and HLA-DRQ1 alleles in Mexican women with oncogenic HPV infection. Japan Journal of Research, 2(3). Retrieved from https://journals.sciencexcel.com/index.php/jjr/article/view/38

Issue

Vol. 2 No. 3

Section

Articles