BMP15/miR-31/FSHR axis regulates yak ovarian granulosa cell proliferation and P4 secretion

Authors

  • Yilong Yao Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Yefen Xu Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research,Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Suolang Sizhu Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Yuheng Wang Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Yun Chen Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangzhou 518000, China
  • Qiangba Yangzong Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Jiaqiang Niu Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research,Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Honghui Wang Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Nyingzhi, Tibet 860000, China
  • Qifa Li Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangzhou 518000, China
  • Linghua Cheng College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
  • Min Guo College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

Keywords:

Yak; GCs; BMP15; FSHR; miR-31; P4; proliferation

Abstract

Background: Bone morphogenetic protein 15 (BMP15) is an oocyte secretory factor expressed mainly
in mammalian oocytes and exerts biological functions through binding to specific receptors on the
granulosa/sheath cell membrane. BMP15 regulates the development and proliferation of granulosa cells
(GCs) and inhibits follicle stimulating hormone (FSH)-dependent cell differentiation and proliferation.
In this report, the regulatory mechanism of BMP15 on yak ovary granulosa cell proliferation and
hormone secretion was examined. Methods: An iodine progesterone radio-immunoassay was used to
detect the secretion of progesterone (P4) in yak granulosa cells. FSH receptor (FSHR) binding miRNAs
were predicted by bioinformatics analysis. The expression of FSHR mRNA and microRNAs was
measured by qPCR, and the expression of FSHR was detected by western blotting. The proliferation
of granulosa cells was measured using the CCK-8 kit. Results: We demonstrate for the first time that
BMP15 downregulates the expression of FSHR and upregulates the expression of microRNA-31 (miR-
31) in yak GCs. Dual luciferase activity results indicate that miR-31 binds directly to the FSHR 3' UTR.
miR-31 was found to promote the proliferation of yak GCs and inhibit the secretion of P4 by inhibiting
the expression of the FSHR in yak GCs. Conclusions: The BMP15/miR-31/FSHR axis identified in this
study is a new mechanism for regulating yak GC function.

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Published

2020-12-12

Issue

Vol. 1 No. 1

Section

Articles