Cytotoxicity of novel plant-based silver nanoparticles on fibroblasts for use in dental and medical applications

Abstract

Background: The aim of this study was to evaluate the cytotoxicity of novel plant-based silver nanoparticles (AgNPs) on mammalian fibroblast cells. Materials and methods: An in vitro prospective, double blind study model was used. A range of 5-17.5 ppm of the novel AgNPs were used in a neutral red uptake cytotoxicity assay. All test samples were compared to a negative and positive control to assess validity. Results: The novel AgNPs showed non-cytotoxicity at all concentrations tested. All tested values were significant compared to the positive controls which showed cytotoxicity (<70% cell viability) via one way anova (p<0.05). Conclusion: The novel AgNPs show promise for use in preventative applications for Dentistry and Medicine. The novel AgNPs could be used as a non-toxic alternative to Chlorhexidine and Cetylpyridinium Chloride