Exploring Genetic Diversity in Enterococcus Faecalis Strains of Endodontic Origin: Pilot Study

Abstract

Objective: The variability in reports associating Enterococcus faecalis with endodontic treatment failure is due to its remarkable genetic plasticity and clonal diversity facilitating the adaptation to adverse microenvironments. Materials and Methods: Present study approximated the genetic diversity of Enterococcus faecalis isolates from oral cavity samples of endodontic origin; the detection involves genes Asa, Esp, EfaA, Ace, Asa373, GelE, and CylA, and mature biofilm formation. Results: On the other side, the genetic clusters were analysed based on the sequence alignment of the amplified product of a 16S rDNA PCR. Results showed that the genetic clustering of Enterococcus faecalis isolates was distinct between patients but with a high degree of similarity between samples from the same individual. Conclusion: The virulence genes presence and biofilm formation resulted independent of genetic clustering but related to sample origin