Influence of the codon 72 polymorphism of the TP53 gene in the susceptibility of female cancers in Widou Thiengoly

Abstract

Genetic factors have been implicated in the predisposition to cancer in Senegalese women. The arginine allele at codon 72 of exon 4 of the TP53 gene has been associated with a predisposition to several cancers in different populations. The objective of this study was to determine the effect of this polymorphism in a Senegalese population located in the locality of Widou Thiengoly. Two hundred and six (206) women living in Widou were recruited as well as thirty-two (32) control women, all assumed to be healthy. Genotyping of codon 72 of exon 4 of the TP53 gene was performed by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) in 122 women. A significant association was found in the distribution of allelic and genotypic frequencies depending on the locality (p value: 5.6 x 10- 09; OR: 0.015; IC95: 3.5e-04-11e-02). However, no correlation was found between the risk factors (age, menopause, oral contraception, parity, gestational age, date of onset of first menstrual period, marital status) and the distribution of the allele frequency in the Widou locality. Due to the strong presence of the Arg allele in the Widou locality, a vast screening campaign for female cancers would be recommended.